Chitosan oligosaccharide seed soaking formula and its effect on mitigating the damage of corn tebuconazole

Chitosan oligosaccharide seed soaking formula and its effect on mitigating the damage of corn tebuconazole

In recent years, the planting area of corn hybrid varieties has continued to increase, and the hair smut disease has increased year by year, especially in the spring corn area in northern China. Using tebuconazole seed coating is one of the most extensive and effective means to prevent and control corn hair smut. However, due to improper dosage during actual use, it is very easy to cause drug damage, and extreme conditions such as low temperature stress aggravate the occurrence of drug damage. Many reports show that triazole fungicides such as tebuconazole inhibit seed germination, causing deformities in the seedling stage and serious economic losses. Chitosan oligosaccharides play the role of important signal molecules in plant resistance. Their role in promoting plant growth by improving carbon and nitrogen assimilation capacity has been proven by many studies. We studied the mitigation effect of chitosan oligosaccharide seed soaking at different concentrations on tebuconazole damage, aiming to provide a theoretical basis for reducing the damage of seed coating agents.

The experiment used chitosan oligosaccharides with mass concentrations of 60, 160, 260, 360, 460, 560, and 660 mg/L, respectively, to soak corn seeds. After drying, they were coated with 60 g/L tebuconazole FS at a coating ratio of 1:300. Two controls were set up, deionized water treatment (CK) and 60 g/L tebuconazole FS treatment (T). The germination test was carried out using the paper culture method, and the changes in various morphological and physiological indicators were statistically analyzed at 3, 4, 5, 6, 7, 8, and 9 days.

Note: The chitosan oligosaccharides used in this experiment were provided by our company yudacentury

The experimental results showed that when the chitosan oligosaccharide was soaked in 360 mg/L, the germination index, vitality index, nutrient transport rate, and α-amylase activity of corn seeds increased by 30.50%, 33.78%, 16.58%, and 27.89% respectively compared with the treatment with 60 g/L tebuconazole FS alone. This indicated that chitosan oligosaccharide treatment had a good alleviating effect on the phytotoxicity of tebuconazole, and the optimal concentration was 360 mg/L.

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