Chitosan oligosaccharide and Tobacco disease

                    Chitosan oligosaccharide and Tobacco disease

Chitosan oligosaccharides are used in tobacco cultivation, which have the functions of preventing root knot nematodes, preventing black shank disease and tobacco mosaic virus, and regulating growth.

Preventing tobacco root knot nematodes

The use of oligosaccharides combined with arbuscular mycorrhizal fungi can inhibit nematode resistance indices such as root knot nematode count, nematode egg count, reproductive index, and root knot index, reducing them by 44.6%, 87.9%, 89.4%, and 79.8%, respectively.

Chitosan oligosaccharides combined with arbuscular mycorrhizal fungi can effectively prevent and control tobacco root knot nematode disease by increasing rhizosphere soil enzyme activity, improving rhizosphere soil microbial population structure, and reducing root knot nematode resistance index; The combination of the two can promote the growth and development of tobacco plants, improve root vitality and nutrient absorption ability, ultimately improve the chemical composition of tobacco leaves and enhance their quality.

Protective effect against low temperature stress

The application of exogenous chitooligosaccharides can reduce oxidative damage to tobacco seedlings under low temperature stress, increase the content of osmoregulatory substances, enhance plant antioxidant activity, and alleviate the damage to tobacco seedlings in low temperature environments. The protective effect of oligosaccharides on tobacco seedlings under low temperature stress provides a good idea for alleviating tobacco's response to low temperature disasters such as late spring cold. Among them, 120mg/L chitosan oligosaccharides had the strongest alleviating effect on tobacco seedlings under low temperature stress; Therefore, in practical production applications, considering efficiency and cost, 100 mg/L chitosan oligosaccharides are the optimal choice.

The role of improving tobacco quality

The combined spraying of chitosan oligosaccharides and salicylic acid solution has a better effect than the separate spraying of the two substances, manifested in the rapid and sustained enhancement of enzyme activity and gene expression, which compensates for the shortcomings of the separate spraying of the two substances. In addition, the treatment group also showed lower levels of malondialdehyde and higher levels of free proline, which had a positive impact on improving tobacco plant resistance. According to the results of incidence rate of each treatment, the treatment group showed the lowest incidence rate, indicating that the joint spraying of the two was more effective in improving the disease resistance of tobacco plants. In addition, the internal chemical composition of the tobacco leaves after sun drying in the treatment group was balanced, and the potassium content was appropriately increased while the chlorine content was reduced, indicating that the single or combined application of chitosan oligosaccharides has a positive impact on the quality of tobacco plants. The reason for the differences in various treatment indicators caused by spraying chitosan oligosaccharides and salicylic acid may be due to the fact that exogenous salicylic acid can directly activate the acquired resistance system as a signaling molecule. Therefore, spraying salicylic acid solution alone has a more rapid effect, but does not show a significant promoting effect on tobacco plant growth. Although the effect of spraying chitosan solution alone lags behind, its effect is more long-lasting and can promote tobacco plant growth through other induction pathways. Recommend using a concentration of 1mmol/L salicylic acid and 110mg/L chitosan.

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